› 7bjs

X-ray diffraction
2.28Å resolution

Crystal structure of Khc/atypical Tm1 complex

Released:
DOI: 10.2210/pdb7bjs/pdb
PDB entry 7bjs coloured by chain, front view.
PDB entry 7bjs coloured by chain, side view.
PDB entry 7bjs coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Molecular basis of mRNA transport by a kinesin-1-atypical tropomyosin complex.
Dimitrova-Paternoga L, Jagtap PKA, Cyrklaff A, Lapouge K, Sehr P, Perez K, Heber S, Löw C, Hennig J, Ephrussi A
Genes Dev 35 976-991 (2021)
PMID: 34140355

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147899 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Kinesin heavy chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 89 amino acids
Theoretical weight: 10.59 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17210 (Residues: 855-941; Coverage: 9%)
Gene names: CG7765, Khc, kin
Tropomyosin 1, isoform E Chain: C
Molecule details ›
Chain: C
Length: 85 amino acids
Theoretical weight: 9.75 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IGY1 (Residues: 477-559; Coverage: 12%)
Gene names: 1305/10, 2299, BcDNA:GH09289, BcDNA:LD37158, BcDNA:SD21996, CG4898, Dm Tm1, Dm TmH33, Dm TmH34, DmTm1, Dmel\CG4898, Dmel_CG4898, PmI, TM, TM1, TMII, Tm, Tm1, TmH, TmH-33, TmH-34, TmH33, TmH34, TmII, Tmr33, Tmr34, TnH, TnH-33, TnH-34, cTM, cTm, cTmII, chr3R:11122272..11122408, l(3)02299, l(3)S130510, l(3)s2958, mTmII, region 3, tm1, tmII, tropomyosin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 28.77Å b: 33.49Å c: 126Å
α: 90° β: 94.58° γ: 90°
R-values:
R R work R free
0.231 0.228 0.278
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

5 review citations

Emerging Roles of RNA-Binding Proteins in Neurodevelopment.
Parra et al. (2022)
4 more

3 mentions without citation

Kinesin-1 captures RNA cargo in its adaptable coils.
Cross et al. (2021)
2 more