› 6yhm

X-ray diffraction
1.13Å resolution

Crystal structure of the C-terminal domain of CNFy from Yersinia pseudotuberculosis

Released:
DOI: 10.2210/pdb6yhm/pdb
PDB entry 6yhm coloured by chain, front view.
PDB entry 6yhm coloured by chain, side view.
PDB entry 6yhm coloured by chain, top view.
Source organism: Yersinia pseudotuberculosis
Primary publication:
Crystal structure of bacterial cytotoxic necrotizing factor CNFY reveals molecular building blocks for intoxication.
Chaoprasid P, Lukat P, Mühlen S, Heidler T, Gazdag EM, Dong S, Bi W, Rüter C, Kirchenwitz M, Steffen A, Jänsch L, Stradal TEB, Dersch P, Blankenfeldt W
EMBO J 40 e105202 (2021)
PMID: 33410511

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102546 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytotoxic necrotizing factor Chain: A
Molecule details ›
Chain: A
Length: 299 amino acids
Theoretical weight: 32.84 KDa
Source organism: Yersinia pseudotuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0N9JNY6 (Residues: 720-1014; Coverage: 29%)
Gene name: cnf
Sequence domains: Rho-activating domain of cytotoxic necrotizing factor

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P21
Unit cell:
a: 41.981Å b: 50.16Å c: 69.728Å
α: 90° β: 98.72° γ: 90°
R-values:
R R work R free
0.168 0.167 0.185
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The Cytotoxic Necrotizing Factors (CNFs)-A Family of Rho GTPase-Activating Bacterial Exotoxins.
Chaoprasid et al. (2021)
1 more

1 mention without citation

Conformational Insights into the Control of CNF1 Toxin Activity by Peptidyl-Prolyl Isomerization: A Molecular Dynamics Perspective.
Paillares et al. (2021)