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An open interface in the pre-80S ribosome coordinated by ribosome assembly factors Tsr1 and Dim1 enables temporal regulation of Fap7.

Rai J, Parker MD, Huang H, Choy S, Ghalei H, Johnson MC, Karbstein K, Stroupe ME
RNA 27 221-233 (2021)
Cited: 13 times
EuropePMC logo PMID: 33219089

Abstract

During their maturation, nascent 40S subunits enter a translation-like quality control cycle, where they are joined by mature 60S subunits to form 80S-like ribosomes. While these assembly intermediates are essential for maturation and quality control, how they form, and how their structure promotes quality control, remains unknown. To address these questions, we determined the structure of an 80S-like ribosome assembly intermediate to an overall resolution of 3.4 Å. The structure, validated by biochemical data, resolves a large body of previously paradoxical data and illustrates how assembly and translation factors cooperate to promote the formation of an interface that lacks many mature subunit contacts but is stabilized by the universally conserved methyltransferase Dim1. We also show how Tsr1 enables this interface by blocking the canonical binding of eIF5B to 40S subunits, while maintaining its binding to 60S. The structure also shows how this interface leads to unfolding of the platform, which allows for temporal regulation of the ATPase Fap7, thus linking 40S maturation to quality control during ribosome assembly.

Reviews - 6wdr mentioned but not cited (1)

  1. Quality control ensures fidelity in ribosome assembly and cellular health. Parker MD, Karbstein K. J Cell Biol 222 e202209115 (2023)

Articles - 6wdr mentioned but not cited (4)

  1. Quality control of 40S ribosome head assembly ensures scanning competence. Huang H, Ghalei H, Karbstein K. J Cell Biol 219 e202004161 (2020)
  2. The modifying enzyme Tsr3 establishes the hierarchy of Rio kinase binding in 40S ribosome assembly. Huang H, Parker M, Karbstein K. RNA 28 568-582 (2022)
  3. research-article A disease associated mutant reveals how Ltv1 orchestrates RP assembly and rRNA folding of the small ribosomal subunit head. Blomqvist EK, Huang H, Karbstein K. bioRxiv 2023.07.10.548325 (2023)
  4. A disease associated mutant reveals how Ltv1 orchestrates RP assembly and rRNA folding of the small ribosomal subunit head. Blomqvist EK, Huang H, Karbstein K. PLoS Genet 19 e1010862 (2023)


Reviews citing this publication (2)

  1. The World of Stable Ribonucleoproteins and Its Mapping With Grad-Seq and Related Approaches. Gerovac M, Vogel J, Smirnov A. Front Mol Biosci 8 661448 (2021)
  2. Genetics animates structure: leveraging genetic interactions to study the dynamics of ribosome biogenesis. Black JJ, Johnson AW. Curr Genet 67 729-738 (2021)

Articles citing this publication (6)

  1. Assembly factors chaperone ribosomal RNA folding by isolating helical junctions that are prone to misfolding. Huang H, Karbstein K. Proc Natl Acad Sci U S A 118 e2101164118 (2021)
  2. CryoEM structures of pseudouridine-free ribosome suggest impacts of chemical modifications on ribosome conformations. Zhao Y, Rai J, Yu H, Li H. Structure 30 983-992.e5 (2022)
  3. The nucleoplasmic phase of pre-40S formation prior to nuclear export. Cheng J, Lau B, Thoms M, Ameismeier M, Berninghausen O, Hurt E, Beckmann R. Nucleic Acids Res 50 11924-11937 (2022)
  4. Release of the ribosome biogenesis factor Bud23 from small subunit precursors in yeast. Black JJ, Johnson AW. RNA 28 371-389 (2022)
  5. Attacking a DEAD problem: The role of DEAD-box ATPases in ribosome assembly and beyond. Karbstein K. Methods Enzymol 673 19-38 (2022)
  6. Using DMS-MaPseq to uncover the roles of DEAD-box proteins in ribosome assembly. Liu X, Huang H, Karbstein K. Methods 204 249-257 (2022)


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