X-ray diffraction
1.86Å resolution

Human CFTR first nucleotide binding domain with dF508/V510D

Source organism: Homo sapiens
Entry authors: Simon KS, Kothe M, Hilbert B, Batchelor JD, Hurlbut GD

Function and Biology Details

Reaction catalysed:
ATP + H(2)O + closed Cl(-) channel = ADP + phosphate + open Cl(-) channel
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Cystic fibrosis transmembrane conductance regulator Chains: A, B
Molecule details ›
Chains: A, B
Length: 226 amino acids
Theoretical weight: 25.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P13569 (Residues: 388-646; Coverage: 15%)
Gene names: ABCC7, CFTR
Sequence domains: ABC transporter

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 62.396Å b: 81.66Å c: 100.404Å
α: 90° β: 90° γ: 90°
R R work R free
0.172 0.17 0.213
Expression system: Escherichia coli BL21(DE3)