› 6uv1

X-ray diffraction
2.31Å resolution

Crystal structure of RNA helicase DDX17 in complex of rU10 RNA

Released:
DOI: 10.2210/pdb6uv1/pdb
PDB entry 6uv1 coloured by chain, front view.
PDB entry 6uv1 coloured by chain, side view.
PDB entry 6uv1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
RNA Specificity and Autoregulation of DDX17, a Modulator of MicroRNA Biogenesis.
Ngo TD, Partin AC, Nam Y
OpenAccess logo Cell Rep 29 4024-4035.e5 (2019)
PMID: 31851931

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187778 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Probable ATP-dependent RNA helicase DDX17 Chains: A, B
Molecule details ›
Chains: A, B
Length: 448 amino acids
Theoretical weight: 51.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92841 (Residues: 111-556; Coverage: 61%)
Gene name: DDX17
Sequence domains:
RNA rU10 Chains: C, D
Molecule details ›
Chains: C, D
Length: 10 nucleotides
Theoretical weight: 3.02 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand ADP 2 x ADP
Ligand BEF 2 x BEF
Ligand MG 10 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 60.752Å b: 91.074Å c: 103.496Å
α: 90° β: 95.56° γ: 90°
R-values:
R R work R free
0.2 0.197 0.255
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

The role of miRNA biogenesis and DDX17 in tumorigenesis and cancer stemness.
Wu KJ. (2020)
8 more

3 mentions without citation

Host DDX Helicases as Possible SARS-CoV-2 Proviral Factors: A Structural Overview of Their Hijacking Through Multiple Viral Proteins.
Squeglia et al. (2020)
2 more