› 6tzk

X-ray diffraction
1.85Å resolution

Bacterial cellulose synthase outermembrane channel BcsC with terminal TPR repeat

Released:
DOI: 10.2210/pdb6tzk/pdb
PDB entry 6tzk coloured by chain, front view.
PDB entry 6tzk coloured by chain, side view.
PDB entry 6tzk coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Architecture of the Cellulose Synthase Outer Membrane Channel and Its Association with the Periplasmic TPR Domain.
Acheson JF, Derewenda ZS, Zimmer J
Structure 27 1855-1861.e3 (2019)
PMID: 31604608

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153455 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cellulose synthase operon protein C Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 50.39 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37650 (Residues: 710-1157; Coverage: 40%)
Gene names: JW5942, b3530, bcsC, yhjL
Sequence domains: Cellulose synthase operon protein C C-terminus (BCSC_C)

Ligands and Environments

6 bound ligands:
Ligand LMT 1 x LMT
Ligand HEZ 1 x HEZ
Ligand PE5 1 x PE5
Ligand C8E 1 x C8E
Ligand 3PE 1 x 3PE
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21212
Unit cell:
a: 87.713Å b: 73.721Å c: 85.777Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.164 0.183
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Role of the lipid bilayer in outer membrane protein folding in Gram-negative bacteria.
Horne et al. (2020)
3 more

5 mentions without citation

Non-canonical autophagy drives alternative ATG8 conjugation to phosphatidylserine.
Durgan et al. (2021)
4 more