› 6tv7

X-ray diffraction
2.9Å resolution

Crystal structure of rsGCaMP in the OFF state (illuminated)

Released:
DOI: 10.2210/pdb6tv7/pdb
PDB entry 6tv7 coloured by chain, front view.
PDB entry 6tv7 coloured by chain, side view.
PDB entry 6tv7 coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Genetically encoded photo-switchable molecular sensors for optoacoustic and super-resolution imaging.
Mishra K, Fuenzalida-Werner JP, Pennacchietti F, Janowski R, Chmyrov A, Huang Y, Zakian C, Klemm U, Testa I, Niessing D, Ntziachristos V, Stiel AC
OpenAccess logo Nat Biotechnol 40 598-605 (2022)
PMID: 34845372

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143908 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calmodulin-1 Chain: A
Molecule details ›
Chain: A
Length: 422 amino acids
Theoretical weight: 47.84 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DP23 (Residues: 3-149; Coverage: 99%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair

Ligands and Environments

2 bound ligands:
Ligand CA 4 x CA
Ligand NA 5 x NA
1 modified residue:
Ligand PIA 1 x PIA

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P41212
Unit cell:
a: 120.67Å b: 120.67Å c: 96.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.182 0.25
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Looking deep inside tissue with photoacoustic molecular probes: a review.
Hui et al. (2022)
1 more