› 6t4v

X-ray diffraction
1.81Å resolution

Crystal structure of 2-methylisocitrate lyase (PrpB) from Pseudomonas aeruginosa in apo form.

Released:
DOI: 10.2210/pdb6t4v/pdb
PDB entry 6t4v coloured by chain, front view.
PDB entry 6t4v coloured by chain, side view.
PDB entry 6t4v coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Entry authors: Wijaya AJ, Brear P, Dolan SK, Welch M

Function and Biology Details

Reaction catalysed: 
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191413 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-methylisocitrate lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 298 amino acids
Theoretical weight: 32.17 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I5E2 (Residues: 1-298; Coverage: 100%)
Gene names: PA0796, prpB
Sequence domains: Phosphoenolpyruvate phosphomutase

Ligands and Environments

1 bound ligand:
Ligand PYR 4 x PYR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 152.859Å b: 59.191Å c: 148.112Å
α: 90° β: 120.08° γ: 90°
R-values:
R R work R free
0.195 0.194 0.216
Expression system: Escherichia coli BL21(DE3)

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