› 6t26

X-ray diffraction
2.26Å resolution

X-ray crystal structure of Vibrio alkaline phosphatase with the non-competitive inhibitor cyclohexylamine

Released:
DOI: 10.2210/pdb6t26/pdb
PDB entry 6t26 coloured by chain, front view.
PDB entry 6t26 coloured by chain, side view.
PDB entry 6t26 coloured by chain, top view.
Source organism: Vibrio sp. G15-21
Primary publication:
X-ray crystal structure of Vibrio alkaline phosphatase with the non-competitive inhibitor cyclohexylamine.
Ásgeirsson B, Markússon S, Hlynsdóttir SS, Helland R, Hjörleifsson JG
Biochem Biophys Rep 24 100830 (2020)
PMID: 33102813

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188109 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alkaline phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 512 amino acids
Theoretical weight: 56.63 KDa
Source organism: Vibrio sp. G15-21
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93P54 (Residues: 20-521; Coverage: 96%)
Sequence domains: Alkaline phosphatase

Ligands and Environments

5 bound ligands:
Ligand ZN 4 x ZN
Ligand MG 6 x MG
Ligand CL 6 x CL
Ligand PO4 2 x PO4
Ligand HAI 4 x HAI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX IV BEAMLINE BioMAX
Spacegroup: P21
Unit cell:
a: 76.916Å b: 85.27Å c: 84.785Å
α: 90° β: 113.55° γ: 90°
R-values:
R R work R free
0.162 0.16 0.206
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Monitoring protein conformational changes using fluorescent nanoantennas.
Harroun et al. (2022)

1 mention without citation

X-ray crystal structure of Vibrio alkaline phosphatase with the non-competitive inhibitor cyclohexylamine.
Ásgeirsson et al. (2020)