› 6sqt

X-ray diffraction
1.84Å resolution

Structure of the U1A variant A1-98 Y31H/Q36R/F56W triple mutant

Released:
DOI: 10.2210/pdb6sqt/pdb
PDB entry 6sqt coloured by chain, front view.
PDB entry 6sqt coloured by chain, side view.
PDB entry 6sqt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Expanding crystallization tools for nucleic acid complexes using U1A protein variants.
Rosenbach H, Victor J, Borggräfe J, Biehl R, Steger G, Etzkorn M, Span I
J Struct Biol 210 107480 (2020)
PMID: 32070773

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140521 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
U1 small nuclear ribonucleoprotein A Chains: AAA, BBB, CCC
Molecule details ›
Chains: AAA, BBB, CCC
Length: 98 amino acids
Theoretical weight: 11.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09012 (Residues: 1-98; Coverage: 35%)
Gene name: SNRPA
Sequence domains: RNA recognition motif

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: P3221
Unit cell:
a: 89.81Å b: 89.81Å c: 86.56Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.213 0.245
Expression system: Escherichia coli

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Citations

2 review citations

Molecular Features and Metal Ions That Influence 10-23 DNAzyme Activity.
Rosenbach et al. (2020)
1 more