› 6s33

X-ray diffraction
1.56Å resolution

Ligand binding domain of the P. putida receptor PcaY_PP in complex with Protocatechuate

Released:
DOI: 10.2210/pdb6s33/pdb
PDB entry 6s33 coloured by chain, front view.
PDB entry 6s33 coloured by chain, side view.
PDB entry 6s33 coloured by chain, top view.
Source organism: Pseudomonas putida KT2440
Primary publication:
The structural basis for signal promiscuity in a bacterial chemoreceptor.
Gavira JA, Matilla MA, Fernández M, Krell T
FEBS J 288 2294-2310 (2021)
PMID: 33021055

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183478 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyl-accepting chemotaxis protein PcaY Chains: A, B
Molecule details ›
Chains: A, B
Length: 550 amino acids
Theoretical weight: 59.54 KDa
Source organism: Pseudomonas putida KT2440
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q88JK6 (Residues: 1-550; Coverage: 100%)
Gene names: PP_2643, pcaY
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ACT 1 x ACT
Ligand DHB 2 x DHB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P212121
Unit cell:
a: 44.886Å b: 67.714Å c: 94.461Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.198
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

2 review citations

The role of solute binding proteins in signal transduction.
Matilla et al. (2021)
1 more

1 mention without citation

The structural basis for signal promiscuity in a bacterial chemoreceptor.
Gavira et al. (2021)