› 6p82

X-ray diffraction
2.05Å resolution

Structure of P. aeruginosa ATCC27853 CdnD, Apo form 1

Released:
DOI: 10.2210/pdb6p82/pdb
PDB entry 6p82 coloured by chain, front view.
PDB entry 6p82 coloured by chain, side view.
PDB entry 6p82 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
HORMA Domain Proteins and a Trip13-like ATPase Regulate Bacterial cGAS-like Enzymes to Mediate Bacteriophage Immunity.
Ye Q, Lau RK, Mathews IT, Birkholz EA, Watrous JD, Azimi CS, Pogliano J, Jain M, Corbett KD
Mol Cell 77 709-722.e7 (2020)
PMID: 31932165

Function and Biology Details

Reaction catalysed: 
2 ATP = 2 diphosphate + cyclic di-3',5'-adenylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145119 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclic AMP-AMP-AMP synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 302 amino acids
Theoretical weight: 34.35 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DTF7 (Residues: 2-300; Coverage: 100%)
Gene names: A4W92_29535, cdnD
Sequence domains: Second Messenger Oligonucleotide or Dinucleotide Synthetase domain

Ligands and Environments

1 bound ligand:
Ligand SO4 34 x SO4
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Unit cell:
a: 104.589Å b: 117.387Å c: 147.804Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.213 0.243
Expression system: Escherichia coli

Quick links

Citations

17 review citations

The ever-expanding world of bacterial cyclic oligonucleotide second messengers.
Yoon et al. (2021)
16 more

2 mentions without citation

HORMA Domain Proteins and a Trip13-like ATPase Regulate Bacterial cGAS-like Enzymes to Mediate Bacteriophage Immunity.
Ye et al. (2020)
1 more