› 6or8

X-ray diffraction
1.65Å resolution

An Unexpected Intermediate in the Reaction Catalyzed by Quinolinate Synthase

Released:
DOI: 10.2210/pdb6or8/pdb
PDB entry 6or8 coloured by chain, front view.
PDB entry 6or8 coloured by chain, side view.
PDB entry 6or8 coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
An Unexpected Species Determined by X-ray Crystallography that May Represent an Intermediate in the Reaction Catalyzed by Quinolinate Synthase.
Esakova OA, Silakov A, Grove TL, Warui DM, Yennawar NH, Booker SJ
J Am Chem Soc 141 14142-14151 (2019)
PMID: 31390192

Function and Biology Details

Reaction catalysed: 
Glycerone phosphate + iminosuccinate = pyridine-2,3-dicarboxylate + 2 H(2)O + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129606 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Quinolinate synthase Chain: A
Molecule details ›
Chain: A
Length: 300 amino acids
Theoretical weight: 34.19 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O57767 (Residues: 1-300; Coverage: 100%)
Gene names: PH0013, nadA
Sequence domains: Quinolinate synthetase A protein

Ligands and Environments

3 bound ligands:
Ligand SF4 1 x SF4
Ligand 5XR 1 x 5XR
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 47.393Å b: 52.527Å c: 53.393Å
α: 90° β: 114.39° γ: 90°
R-values:
R R work R free
0.169 0.166 0.208
Expression system: Escherichia coli

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Citations

2 citation in other articles

Heme biosynthesis depends on previously unrecognized acquisition of iron-sulfur cofactors in human amino-levulinic acid dehydratase.
Liu et al. (2020)
1 more