X-ray diffraction
2.05Å resolution

Crystal structure of HPK1 kinase domain T165E,S171E phosphomimetic mutant in complex with sunitinib in the inactive state.


Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Mitogen-activated protein kinase kinase kinase kinase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 309 amino acids
Theoretical weight: 34.5 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
  • Canonical: Q92918 (Residues: 1-307; Coverage: 37%)
Gene names: HPK1, MAP4K1
Sequence domains: Protein kinase domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P1
Unit cell:
a: 55.81Å b: 58.92Å c: 60.93Å
α: 82.44° β: 82.31° γ: 64.34°
R R work R free
0.202 0.201 0.229
Expression system: Spodoptera frugiperda