› 6h2i

X-ray diffraction
2.72Å resolution

Structure of BlaC from Mycobacterium tuberculosis bound to the trans-enamine adduct of tazobactam.

Released:
DOI: 10.2210/pdb6h2i/pdb
PDB entry 6h2i coloured by chain, front view.
PDB entry 6h2i coloured by chain, side view.
PDB entry 6h2i coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
New Conformations of Acylation Adducts of Inhibitors of β-Lactamase from Mycobacterium tuberculosis.
Tassoni R, Blok A, Pannu NS, Ubbink M
Biochemistry 58 997-1009 (2019)
PMID: 30632739

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166805 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chains: A, B
Molecule details ›
Chains: A, B
Length: 274 amino acids
Theoretical weight: 29.48 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand TBE 2 x TBE
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P1
Unit cell:
a: 39.632Å b: 41.523Å c: 76.417Å
α: 103.83° β: 89.99° γ: 90.79°
R-values:
R R work R free
0.206 0.203 0.265
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 citation in other articles

Observation of substrate diffusion and ligand binding in enzyme crystals using high-repetition-rate mix-and-inject serial crystallography.
Pandey et al. (2021)
7 more

2 mentions without citation

New Conformations of Acylation Adducts of Inhibitors of β-Lactamase from Mycobacterium tuberculosis.
Tassoni et al. (2019)
1 more