› 6bq5

X-ray diffraction
1.8Å resolution

Crystal structure of Medicago truncatula Thermospermine Synthase (MtTSPS) in complex with 5'-methylthioadenosine

Released:
DOI: 10.2210/pdb6bq5/pdb
PDB entry 6bq5 coloured by chain, front view.
PDB entry 6bq5 coloured by chain, side view.
PDB entry 6bq5 coloured by chain, top view.
Source organism: Medicago truncatula
Primary publication:
Crystal structure of thermospermine synthase from Medicago truncatula and substrate discriminatory features of plant aminopropyltransferases.
Sekula B, Dauter Z
Biochem J 475 787-802 (2018)
PMID: 29367265

Function and Biology Details

Reaction catalysed: 
S-adenosyl 3-(methylthio)propylamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124578 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PABS domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 331 amino acids
Theoretical weight: 37.28 KDa
Source organism: Medicago truncatula
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G7K2D1 (Residues: 1-328; Coverage: 100%)
Gene names: 11439099, MTR_5g006140, MtrunA17_Chr5g0394371
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand B3P 2 x B3P
Ligand MTA 2 x MTA
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43212
Unit cell:
a: 82.766Å b: 82.766Å c: 164.194Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.176 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Polyamines in Microalgae: Something Borrowed, Something New.
Lin et al. (2018)
5 other articles

3 mentions without citation

Disentangling detoxification: gene expression analysis of feeding mountain pine beetle illuminates molecular-level host chemical defense detoxification mechanisms.
Robert et al. (2013)
2 more