› 6bnd

X-ray diffraction
1.66Å resolution

Crystal structure of the intrinsic colistin resistance enzyme ICR(Mc) from Moraxella catarrhalis, catalytic domain, Thr315Ala mutant mono-zinc and phosphoethanolamine complex

Released:
DOI: 10.2210/pdb6bnd/pdb
PDB entry 6bnd coloured by chain, front view.
PDB entry 6bnd coloured by chain, side view.
PDB entry 6bnd coloured by chain, top view.
Source organism: Moraxella sp. HMSC061H09
Primary publication:
Substrate Recognition by a Colistin Resistance Enzyme from Moraxella catarrhalis.
Stogios PJ, Cox G, Zubyk HL, Evdokimova E, Wawrzak Z, Wright GD, Savchenko A
ACS Chem Biol 13 1322-1332 (2018)
PMID: 29631403

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103443 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoethanolamine transferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 344 amino acids
Theoretical weight: 37.94 KDa
Source organism: Moraxella sp. HMSC061H09
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1E9VP98 (Residues: 235-578; Coverage: 60%)
Gene name: HMPREF2573_04170
Sequence domains: Sulfatase
Structure domains: Alkaline Phosphatase, subunit A

Ligands and Environments

4 bound ligands:
Ligand ZN 2 x ZN
Ligand OPE 2 x OPE
Ligand SO4 1 x SO4
Ligand 15P 3 x 15P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21212
Unit cell:
a: 74.52Å b: 155.24Å c: 66.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.151 0.192
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

Overcoming Fungal Echinocandin Resistance through Inhibition of the Non-essential Stress Kinase Yck2.
Caplan et al. (2020)
6 more

2 mentions without citation

Phosphoethanolamine Transferases as Drug Discovery Targets for Therapeutic Treatment of Multi-Drug Resistant Pathogenic Gram-Negative Bacteria.
Thai et al. (2023)
1 more