› 6yvh

X-ray diffraction
3.19Å resolution

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-153772 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Pre-mRNA-splicing factor CWC22 homolog Chains: A, B, D, F
Molecule details ›
Chains: A, B, D, F
Length: 291 amino acids
Theoretical weight: 33.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HCG8 (Residues: 119-406; Coverage: 32%)
Gene names: CWC22, KIAA1604, NCM
Sequence domains: MIF4G domain
Spliceosome-associated protein CWC27 homolog Chains: C, E, G, I
Molecule details ›
Chains: C, E, G, I
Length: 57 amino acids
Theoretical weight: 6.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6UX04 (Residues: 378-431; Coverage: 11%)
Gene names: CWC27, SDCCAG10, UNQ438/PRO871
Eukaryotic initiation factor 4A-III, N-terminally processed Chains: H, J, K, L
Molecule details ›
Chains: H, J, K, L
Length: 166 amino acids
Theoretical weight: 19.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38919 (Residues: 246-411; Coverage: 40%)
Gene names: DDX48, EIF4A3, KIAA0111
Sequence domains: Helicase conserved C-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 151.255Å b: 163.627Å c: 181.131Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.232 0.263
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

A Day in the Life of the Exon Junction Complex.
Schlautmann et al. (2020)
4 more