› 6x5q

X-ray diffraction
2.14Å resolution

Cocrystal structure of human CaMKII-alpha (CAMK2A)kinase domain and GluA1

Released:
DOI: 10.2210/pdb6x5q/pdb
PDB entry 6x5q coloured by chain, front view.
PDB entry 6x5q coloured by chain, side view.
PDB entry 6x5q coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
CaMKII binds both substrates and activators at the active site.
Özden C, Sloutsky R, Mitsugi T, Santos N, Agnello E, Gaubitz C, Foster J, Lapinskas E, Esposito EA, Saneyoshi T, Kelch BA, Garman SC, Hayashi Y, Stratton MM
Cell Rep 40 111064 (2022)
PMID: 35830796

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154588 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calcium/calmodulin-dependent protein kinase type II subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 268 amino acids
Theoretical weight: 30.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UQM7 (Residues: 7-274; Coverage: 56%)
Gene names: CAMK2A, CAMKA, KIAA0968
Sequence domains: Protein kinase domain
Glutamate receptor 1 Chain: B
Molecule details ›
Chain: B
Length: 20 amino acids
Theoretical weight: 2.32 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P42261 (Residues: 836-855; Coverage: 2%)
Gene names: GLUA1, GLUH1, GLUR1, GRIA1

Ligands and Environments

1 bound ligand:
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 42.629Å b: 57.456Å c: 107.707Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.189 0.235
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

CaMKII: a central molecular organizer of synaptic plasticity, learning and memory.
Yasuda et al. (2022)
2 more

2 mentions without citation

Native Mass Spectrometry-Based Screening for Optimal Sample Preparation in Single-Particle Cryo-EM.
Olinares et al. (2021)
1 more