› 6gsa

Electron Microscopy
4.2Å resolution

Core Centromere Binding Factor 3 (CBF3) with monomeric Ndc10

Released:
DOI: 10.2210/pdb6gsa/pdb
PDB entry 6gsa coloured by chain, front view.
PDB entry 6gsa coloured by chain, side view.
PDB entry 6gsa coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Insights into Centromere DNA Bending Revealed by the Cryo-EM Structure of the Core Centromere Binding Factor 3 with Ndc10.
Zhang W, Lukoyanova N, Miah S, Lucas J, Vaughan CK
Cell Rep 24 744-754 (2018)
PMID: 30021170
Related structures: EMD-0051

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-152338 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Centromere DNA-binding protein complex CBF3 subunit B Chains: A, B
Molecule details ›
Chains: A, B
Length: 584 amino acids
Theoretical weight: 68.45 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P40969 (Residues: 47-608; Coverage: 92%)
Gene names: CBF3, CBF3B, CEP3, CSL1, YM8520.17C, YMR168C
Sequence domains: Centromere DNA-binding protein complex CBF3 subunit B
Suppressor of kinetochore protein 1 Chain: C
Molecule details ›
Chain: C
Length: 197 amino acids
Theoretical weight: 22.56 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P52286 (Residues: 2-194; Coverage: 100%)
Gene names: CBF3D, D9798.14, SKP1, YDR328C
Sequence domains:
Centromere DNA-binding protein complex CBF3 subunit C Chain: D
Molecule details ›
Chain: D
Length: 519 amino acids
Theoretical weight: 60.9 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P35203 (Residues: 2-478; Coverage: 100%)
Gene names: CBF3C, CTF13, YM6543.01, YM9582.19, YMR094W
Centromere DNA-binding protein complex CBF3 subunit A Chain: E
Molecule details ›
Chain: E
Length: 562 amino acids
Theoretical weight: 66.16 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32504 (Residues: 2-553; Coverage: 58%)
Gene names: CBF2, CBF3A, CEP2, CTF14, NDC10, YGR140W
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-0051
Expression system: Saccharomyces cerevisiae

Quick links

Citations

2 review citations

Mechanical Flexibility of DNA: A Quintessential Tool for DNA Nanotechnology.
Saran et al. (2020)
1 more

2 mentions without citation

Seeing is believing: our evolving view of kinetochore structure, composition, and assembly.
Hamilton et al. (2019)
1 more