› 6a6i

X-ray diffraction
2.6Å resolution

Crystal structure of the winged-helix domain of Cockayne syndrome group B protein in complex with ubiquitin

Released:
DOI: 10.2210/pdb6a6i/pdb
PDB entry 6a6i coloured by chain, front view.
PDB entry 6a6i coloured by chain, side view.
PDB entry 6a6i coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of ubiquitin recognition by the winged-helix domain of Cockayne syndrome group B protein.
Takahashi TS, Sato Y, Yamagata A, Goto-Ito S, Saijo M, Fukai S
Nucleic Acids Res 47 3784-3794 (2019)
PMID: 30753618

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143273 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Excision repair cross-complementing rodent repair deficiency, complementation group 6 variant Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 98 amino acids
Theoretical weight: 11.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q59FF6 (Residues: 778-870; Coverage: 11%)
Ubiquitin Chains: B, D
Molecule details ›
Chains: B, D
Length: 76 amino acids
Theoretical weight: 8.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG47 (Residues: 153-228; Coverage: 33%)
Gene name: UBB
Sequence domains: Ubiquitin family
Ubiquitin Chains: F, H
Molecule details ›
Chains: F, H
Length: 77 amino acids
Theoretical weight: 8.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG47 (Residues: 77-153; Coverage: 34%)
Gene name: UBB
Sequence domains: Ubiquitin family

Ligands and Environments

2 bound ligands:
Ligand GOL 3 x GOL
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 54.161Å b: 101.705Å c: 66.008Å
α: 90° β: 101.81° γ: 90°
R-values:
R R work R free
0.181 0.178 0.236
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Current and emerging roles of Cockayne syndrome group B (CSB) protein.
Tiwari et al. (2021)
2 more

6 mentions without citation

Cockayne Syndrome Group B (CSB): The Regulatory Framework Governing the Multifunctional Protein and Its Plausible Role in Cancer.
Spyropoulou et al. (2021)
5 more