› 5vn4

X-ray diffraction
1.35Å resolution

Crystal structure of adenine phosphoribosyl transferase from Trypanosoma brucei in complex with AMP, pyrophosphate, and ribose-5-phosphate

Released:
DOI: 10.2210/pdb5vn4/pdb
PDB entry 5vn4 coloured by chain, front view.
PDB entry 5vn4 coloured by chain, side view.
PDB entry 5vn4 coloured by chain, top view.
Source organism: Trypanosoma brucei brucei TREU927
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176530 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
adenine phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 243 amino acids
Theoretical weight: 27.04 KDa
Source organism: Trypanosoma brucei brucei TREU927
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q57V32 (Residues: 1-235; Coverage: 100%)
Gene names: Tb07.43M14.180, Tb927.7.1780
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

4 bound ligands:
Ligand ADE 2 x ADE
Ligand HSX 2 x HSX
Ligand PPV 2 x PPV
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 72.84Å b: 72.85Å c: 85.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.144 0.143 0.175
Expression system: Escherichia coli BL21(DE3)

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