› 5tpv

X-ray diffraction
2.14Å resolution

X-ray structure of WlaRA (TDP-fucose-3,4-ketoisomerase) from Campylobacter jejuni

Released:
DOI: 10.2210/pdb5tpv/pdb
PDB entry 5tpv coloured by chain, front view.
PDB entry 5tpv coloured by chain, side view.
PDB entry 5tpv coloured by chain, top view.
Source organism: Campylobacter jejuni subsp. jejuni 81116
Primary publication:
Characterization of the dTDP-Fuc3N and dTDP-Qui3N biosynthetic pathways in Campylobacter jejuni 81116.
Li ZZ, Riegert AS, Goneau MF, Cunningham AM, Vinogradov E, Li J, Schoenhofen IC, Thoden JB, Holden HM, Gilbert M
Glycobiology 27 358-369 (2017)
PMID: 28096310

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189584 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sugar 3,4-ketoisomerase QdtA cupin domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 153 amino acids
Theoretical weight: 18.39 KDa
Source organism: Campylobacter jejuni subsp. jejuni 81116
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ALS3 (Residues: 1-145; Coverage: 100%)
Sequence domains: WxcM-like, C-terminal
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand TYD 3 x TYD
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: Excillum MetalJet D2+ 160 kV
Spacegroup: I422
Unit cell:
a: 177.953Å b: 177.953Å c: 88.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.207 0.263
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Elucidation of the O-antigen structure of Escherichia coli O63.
Ståhle et al. (2019)
2 more

1 mention without citation

Characterization of the dTDP-Fuc3N and dTDP-Qui3N biosynthetic pathways in Campylobacter jejuni 81116.
Li et al. (2017)