› 5ofr

X-ray diffraction
3.4Å resolution

Structure of the antibacterial peptide ABC transporter McjD in a high energy outward occluded intermediate state

Released:
DOI: 10.2210/pdb5ofr/pdb
PDB entry 5ofr coloured by chain, front view.
PDB entry 5ofr coloured by chain, side view.
PDB entry 5ofr coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis for antibacterial peptide self-immunity by the bacterial ABC transporter McjD.
Bountra K, Hagelueken G, Choudhury HG, Corradi V, El Omari K, Wagner A, Mathavan I, Zirah S, Yuan Wahlgren W, Tieleman DP, Schiemann O, Rebuffat S, Beis K
OpenAccess logo EMBO J 36 3062-3079 (2017)
PMID: 28864543

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194904 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Microcin-J25 export ATP-binding/permease protein McjD Chains: A, B
Molecule details ›
Chains: A, B
Length: 580 amino acids
Theoretical weight: 65.48 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli str. K-12 substr. MG1655
UniProt:
  • Canonical: Q9X2W0 (Residues: 1-580; Coverage: 100%)
Gene name: mcjD
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand ADP 2 x ADP
Ligand MG 2 x MG
Ligand VO4 2 x VO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I23
Spacegroup: C2
Unit cell:
a: 235.29Å b: 105.04Å c: 117.37Å
α: 90° β: 105.55° γ: 90°
R-values:
R R work R free
0.257 0.257 0.26
Expression system: Escherichia coli str. K-12 substr. MG1655

Quick links

Citations

13 review citations

Multidrug efflux pumps: structure, function and regulation.
Du et al. (2018)
12 more

7 mentions without citation

Structural and functional diversity calls for a new classification of ABC transporters.
Thomas et al. (2020)
6 more