› 5mu9

X-ray diffraction
1.3Å resolution

MOA-E-64 complex

Released:
DOI: 10.2210/pdb5mu9/pdb
PDB entry 5mu9 coloured by chain, front view.
PDB entry 5mu9 coloured by chain, side view.
PDB entry 5mu9 coloured by chain, top view.
Source organism: Marasmius oreades
Primary publication:
Family of Papain-Like Fungal Chimerolectins with Distinct Ca2+-Dependent Activation Mechanism.
Cordara G, Manna D, Krengel U
Biochemistry 56 4689-4700 (2017)
PMID: 28665586

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186825 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Agglutinin Chain: A
Molecule details ›
Chain: A
Length: 293 amino acids
Theoretical weight: 32.3 KDa
Source organism: Marasmius oreades
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8X123 (Residues: 1-293; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GAL, FUC, GLA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: FUC, GLA
Carbohydrate polymer
5 bound ligands:
Ligand EDO 1 x EDO
Ligand ACT 1 x ACT
Ligand E64 1 x E64
Ligand CA 3 x CA
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P6322
Unit cell:
a: 120.934Å b: 120.934Å c: 99.851Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.148 0.146 0.183
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 mentions without citation

Identification and classification of papain-like cysteine proteinases.
Ozhelvaci et al. (2023)
1 more