› 5leo

X-ray diffraction
1.6Å resolution

Complex structure of lysostaphin SH3b domain with peptidoglycan fragment

Released:
DOI: 10.2210/pdb5leo/pdb
PDB entry 5leo coloured by chain, front view.
PDB entry 5leo coloured by chain, side view.
PDB entry 5leo coloured by chain, top view.
Source organism: Staphylococcaceae
Entry authors: Jagielska E, Nowak E, Bochtler M, Sabala I

Function and Biology Details

Reaction catalysed: 
Hydrolysis of the -Gly-|-Gly- bond in the pentaglycine inter-peptide link joining staphylococcal cell wall peptidoglycans.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145337 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Lysostaphin Chains: A, B
Molecule details ›
Chains: A, B
Length: 94 amino acids
Theoretical weight: 10.57 KDa
Source organism: Staphylococcaceae
Expression system: Escherichia coli
UniProt:
  • Canonical: P10547 (Residues: 401-493; Coverage: 20%)
Gene name: lss
Sequence domains: Bacterial SH3 domain
Structure domains: SH3 Domains
GLY-GLY-GLY-GLY-GLY Chains: G, H
Molecule details ›
Chains: G, H
Length: 5 amino acids
Theoretical weight: 303 Da
Source organism: Staphylococcaceae
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand SO4 6 x SO4
Ligand MPD 2 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 50.38Å b: 54.364Å c: 63.229Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.184 0.235
Expression systems:
  • Escherichia coli
  • Not provided

Quick links