› 5lb1

X-ray diffraction
1.55Å resolution

Crystal structure of the Mycobacterium tuberculosis L,D-transpeptidase-2 (LdtMt2) BC-module with thionitrobenzoate (TNB) adduct at the active site cysteine-354

Released:
DOI: 10.2210/pdb5lb1/pdb
PDB entry 5lb1 coloured by chain, front view.
PDB entry 5lb1 coloured by chain, side view.
PDB entry 5lb1 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Binding and processing of β-lactam antibiotics by the transpeptidase LdtMt2 from Mycobacterium tuberculosis.
Steiner EM, Schneider G, Schnell R
FEBS J 284 725-741 (2017)
PMID: 28075068

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125201 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L,D-transpeptidase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 262 amino acids
Theoretical weight: 28.51 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: I6Y9J2 (Residues: 149-408; Coverage: 70%)
Gene names: P425_02624, RVBD_2518c, Rv2518c, ldtB, lppS
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MNB 2 x MNB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P1
Unit cell:
a: 53.368Å b: 53.91Å c: 57.174Å
α: 68.84° β: 75.03° γ: 88.64°
R-values:
R R work R free
0.15 0.147 0.194
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Mycobacterium abscessus and β-Lactams: Emerging Insights and Potential Opportunities.
Story-Roller et al. (2018)
3 more

2 mentions without citation

Binding and processing of β-lactam antibiotics by the transpeptidase LdtMt2 from Mycobacterium tuberculosis.
Steiner et al. (2017)
1 more