› 5j2y

X-ray diffraction
2.4Å resolution

Molecular insight into the regulatory mechanism of the quorum-sensing repressor RsaL in Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb5j2y/pdb
PDB entry 5j2y coloured by chain, front view.
PDB entry 5j2y coloured by chain, side view.
PDB entry 5j2y coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of Pseudomonas aeruginosa RsaL bound to promoter DNA reaffirms its role as a global regulator involved in quorum-sensing.
Kang H, Gan J, Zhao J, Kong W, Zhang J, Zhu M, Li F, Song Y, Qin J, Liang H
OpenAccess logo Nucleic Acids Res 45 699-710 (2017)
PMID: 27924027

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118856 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Virulence gene repressor RsaL Chains: A, B
Molecule details ›
Chains: A, B
Length: 80 amino acids
Theoretical weight: 9.5 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9X7H4 (Residues: 1-80; Coverage: 100%)
Gene names: CAZ10_08125, DT376_05895, GNQ48_02435, GUL26_07215, IPC1295_03850, IPC737_12500, L4V69_23650, rsaL
Sequence domains: RsaL-like HTH domain
DNA (26-MER) Chains: F, f
Molecule details ›
Chains: F, f
Length: 26 nucleotides
Theoretical weight: 8 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (26-MER) Chains: R, r
Molecule details ›
Chains: R, r
Length: 26 nucleotides
Theoretical weight: 7.97 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P21212
Unit cell:
a: 97.245Å b: 52.983Å c: 69.809Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.224 0.252
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

5 review citations

Pseudomonas aeruginosa: pathogenesis, virulence factors, antibiotic resistance, interaction with host, technology advances and emerging therapeutics.
Qin et al. (2022)
4 more

5 mentions without citation

Crystal structure of Pseudomonas aeruginosa RsaL bound to promoter DNA reaffirms its role as a global regulator involved in quorum-sensing.
Kang et al. (2017)
4 more