5gw8 › Citations

Malassezia globosa MgMDL2 lipase: Crystal structure and rational modification of substrate specificity.

Lan D, Xu H, Xu J, Dubin G, Liu J, Iqbal Khan F, Wang Y
Biochem Biophys Res Commun 488 259-265 (2017)
Cited: 6 times
EuropePMC logo PMID: 28433636

Abstract

Lipases play an important role in physiological metabolism and diseases, and also have multiple industrial applications. Rational modification of lipase specificity may increase the commercial utility of this group of enzymes, but is hindered by insufficient mechanistic understanding. Here, we report the 2.0 Å resolution crystal structure of a mono- and di-acylglycerols lipase from Malassezia globosa (MgMDL2). Interestingly, residues Phe278 and Glu282 were found to involve in substrate recognition because mutation on each residue led to convert MgMDL2 to a triacylglycerol (TAG) lipase. The Phe278Ala and Glu282Ala mutants also acquired ability to synthesize TAGs by esterification of glycerol and fatty acids. By in silicon analysis, steric hindrance of these residues seemed to be key factors for the altered substrate specificity. Our work may shed light on understanding the unique substrate selectivity mechanism of mono- and di-acylglycerols lipases, and provide a new insight for engineering biocatalysts with desired catalytic behaviors for biotechnological application.

Reviews citing this publication (3)

  1. Skin Commensal Fungus Malassezia and Its Lipases. Park M, Park S, Jung WH. J Microbiol Biotechnol 31 637-644 (2021)
  2. Anti-Malassezia Drug Candidates Based on Virulence Factors of Malassezia-Associated Diseases. Billamboz M, Jawhara S. Microorganisms 11 2599 (2023)
  3. N-Amidation of Nitrogen-Containing Heterocyclic Compounds: Can We Apply Enzymatic Tools? Yang A, Miao X, Yang L, Xu C, Liu W, Xian M, Zou H. Bioengineering (Basel) 10 222 (2023)

Articles citing this publication (3)

  1. Seed Extract of Psoralea corylifolia and Its Constituent Bakuchiol Impairs AHL-Based Quorum Sensing and Biofilm Formation in Food- and Human-Related Pathogens. Husain FM, Ahmad I, Khan FI, Al-Shabib NA, Baig MH, Hussain A, Rehman MT, Alajmi MF, Lobb KA. Front Cell Infect Microbiol 8 351 (2018)
  2. Paradoxical Activation of a Type VI Secretion System Phospholipase Effector by Its Cognate Immunity Protein. Jensen SJ, Ruhe ZC, Williams AF, Nhan DQ, Garza-Sánchez F, Low DA, Hayes CS. J Bacteriol 205 e0011323 (2023)
  3. Production and Quantification of Virulence Factors in Malassezia Species. Hadrich I, Khemekhem N, Neji S, Trablesi H, Ilahi A, Sellami H, Makni F, Ayadi A. Pol J Microbiol 71 529-538 (2022)


Quick links