5ft1

X-ray diffraction
2.75Å resolution

Crystal structure of gp37(Dip) from bacteriophage phiKZ bound to RNase E of Pseudomonas aeruginosa

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-186075 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PHIKZ037 Chains: A, B, E, G, I, K
Molecule details ›
Chains: A, B, E, G, I, K
Length: 281 amino acids
Theoretical weight: 31.99 KDa
Source organism: Phikzvirus phiKZ
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8SDC5 (Residues: 1-273; Coverage: 100%)
Sequence domains: gp37/Dip protein
Ribonuclease E Chains: C, D, F, H, J, L
Molecule details ›
Chains: C, D, F, H, J, L
Length: 20 amino acids
Theoretical weight: 2.7 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Not provided
UniProt:
  • Canonical: Q9HZM8 (Residues: 756-775; Coverage: 2%)
Gene names: PA2976, rne

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P1
Unit cell:
a: 84.61Å b: 84.65Å c: 84.6Å
α: 107.86° β: 107.88° γ: 107.9°
R-values:
R R work R free
0.266 0.263 0.293
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided