› 5h28

X-ray diffraction
1.5Å resolution

Crystal structure of Osh1 ANK domain from Saccharomyces cerevisia

Released:
DOI: 10.2210/pdb5h28/pdb
PDB entry 5h28 coloured by chain, front view.
PDB entry 5h28 coloured by chain, side view.
PDB entry 5h28 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structure of Yeast OSBP-Related Protein Osh1 Reveals Key Determinants for Lipid Transport and Protein Targeting at the Nucleus-Vacuole Junction.
Manik MK, Yang H, Tong J, Im YJ
Structure 25 617-629.e3 (2017)
PMID: 28319008

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153047 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxysterol-binding protein homolog 1 Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 30.05 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35845 (Residues: 12-274; Coverage: 22%)
Gene names: OSH1, SWH1, YAR042W, YAR044W
Sequence domains: Ankyrin repeat

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P21
Unit cell:
a: 29.465Å b: 72.202Å c: 49.008Å
α: 90° β: 93.53° γ: 90°
R-values:
R R work R free
0.184 0.184 0.214
Expression system: Escherichia coli BL21(DE3)

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Citations

9 review citations

The Oxysterol-Binding Protein Cycle: Burning Off PI(4)P to Transport Cholesterol.
Antonny et al. (2018)
8 more