› 5e16

X-ray diffraction
1.65Å resolution

Co-crystal structure of the N-termial cGMP binding domain of Plasmodium falciparum PKG with cGMP

Released:
DOI: 10.2210/pdb5e16/pdb
PDB entry 5e16 coloured by chain, front view.
PDB entry 5e16 coloured by chain, side view.
PDB entry 5e16 coloured by chain, top view.
Source organism: Plasmodium falciparum
Primary publication:
Structures of the cGMP-dependent protein kinase in malaria parasites reveal a unique structural relay mechanism for activation.
El Bakkouri M, Kouidmi I, Wernimont AK, Amani M, Hutchinson A, Loppnau P, Kim JJ, Flueck C, Walker JR, Seitova A, Senisterra G, Kakihara Y, Kim C, Blackman MJ, Calmettes C, Baker DA, Hui R
Proc Natl Acad Sci U S A 116 14164-14173 (2019)
PMID: 31239348

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184718 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cGMP-dependent protein kinase Chain: A
Molecule details ›
Chain: A
Length: 143 amino acids
Theoretical weight: 16.08 KDa
Source organism: Plasmodium falciparum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8I719 (Residues: 21-162; Coverage: 17%)
Gene names: PF3D7_1436600, PKG
Sequence domains: Cyclic nucleotide-binding domain
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand PCG 1 x PCG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: C2221
Unit cell:
a: 51.266Å b: 53.847Å c: 92.351Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.196 0.227
Expression system: Escherichia coli

Quick links

Citations

6 review citations

cGMP: a unique 2nd messenger molecule - recent developments in cGMP research and development.
Friebe et al. (2020)
5 more

4 mentions without citation

Cyclic nucleotide selectivity of protein kinase G isozymes.
Kim et al. (2021)
3 more