› 4yah

X-ray diffraction
1.6Å resolution

Crystal Structure of the Methionine Binding Protein, MetQ

Released:
DOI: 10.2210/pdb4yah/pdb
PDB entry 4yah coloured by chain, front view.
PDB entry 4yah coloured by chain, side view.
PDB entry 4yah coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
The contribution of methionine to the stability of the Escherichia coli MetNIQ ABC transporter-substrate binding protein complex.
Nguyen PT, Li QW, Kadaba NS, Lai JY, Yang JG, Rees DC
Biol Chem 396 1127-34 (2015)
PMID: 25803078

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151256 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-methionine-binding lipoprotein MetQ Chain: X
Molecule details ›
Chain: X
Length: 279 amino acids
Theoretical weight: 30.53 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P28635 (Residues: 1-271; Coverage: 100%)
Gene names: JW0193, b0197, metQ, yaeC
Sequence domains: NlpA lipoprotein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

1 bound ligand:
Ligand MET 1 x MET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: C2221
Unit cell:
a: 59.65Å b: 87.37Å c: 112.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.154 0.183
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 citation in other articles

Profiling the Escherichia coli membrane protein interactome captured in Peptidisc libraries.
Carlson et al. (2019)
5 more

4 mentions without citation

MetQ of Neisseria gonorrhoeae Is a Surface-Expressed Antigen That Elicits Bactericidal and Functional Blocking Antibodies.
Semchenko et al. (2017)
3 more