4v1s

X-ray diffraction
1.5Å resolution

Structure of the GH76 alpha-mannanase BT2949 from Bacteroides thetaiotaomicron

Released:

Function and Biology Details

Reaction catalysed:
Random hydrolysis of (1->6)-alpha-D-mannosidic linkages in unbranched (1->6)-mannans
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative alpha-1,6-mannanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 396 amino acids
Theoretical weight: 45.25 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8A3K5 (Residues: 1-396; Coverage: 100%)
Gene name: BT_2949
Sequence domains: Glycosyl hydrolase family 76
Structure domains: Glycosyltransferase

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Unit cell:
a: 81.677Å b: 121.03Å c: 125.604Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.149 0.18
Expression system: Escherichia coli BL21