X-ray diffraction
1.38Å resolution

Crystal structure of Arabidopsis thaliana ThiC with bound imidazole ribonucleotide and Fe


Function and Biology Details

Reaction catalysed:
5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine = 4-amino-2-methyl-5-(phosphomethyl)pyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Phosphomethylpyrimidine synthase, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 576 amino acids
Theoretical weight: 64.67 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
  • Canonical: O82392 (Residues: 72-644; Coverage: 89%)
Gene names: At2g29630, PY, T27A16.27, THIC
Sequence domains: Radical SAM ThiC family

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P3221
Unit cell:
a: 107.292Å b: 107.292Å c: 87.845Å
α: 90° β: 90° γ: 120°
R R work R free
0.12 0.119 0.143
Expression system: Escherichia coli BL21(DE3)