› 4s0f

X-ray diffraction
5.52Å resolution

Crystal structure of the peptidase-containing ABC transporter PCAT1 E648Q mutant complexed with ATPgS in an occluded conformation

Released:
DOI: 10.2210/pdb4s0f/pdb
PDB 4s0f coloured by chain and viewed from the front.
PDB 4s0f coloured by chain and viewed from the side.
PDB 4s0f coloured by chain and viewed from the top.
Source organism: Acetivibrio thermocellus ATCC 27405
Primary publication:
Crystal structures of a polypeptide processing and secretion transporter.
Lin DY, Huang S, Chen J
Nature 523 425-30 (2015)
PMID: 26201595

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ABC-type bacteriocin transporter Chains: A, B
Molecule details ›
Chains: A, B
Length: 730 amino acids
Theoretical weight: 81.18 KDa
Source organism: Acetivibrio thermocellus ATCC 27405
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A3DCU1 (Residues: 1-727; Coverage: 100%)
Gene name: Cthe_0534
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand AGS 2 x AGS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P42212
Unit cell:
a: 230Å b: 230Å c: 89.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.301 0.3 0.314
Expression system: Escherichia coli BL21(DE3)

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Citations

10 review citations

Recent Insights into the Structure and Function of Mycobacterial Membrane Proteins Facilitated by Cryo-EM.
Bendre et al. (2021)
9 more

7 mentions without citation

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Alav et al. (2021)
6 more