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Streptomyces coelicolor SCO4226 is a nickel binding protein.

<div class='caption-title'>Overall structures of SCO4226 in (A) apo- and (B) nickel-bound forms (pink and cyan for each subunit, respectively).</div>
<div class='caption-title'>The coordination patterns of (A) Ni-1, (B) Ni-2, (C) Ni-3 and (D) Ni-4.</div>
<div class='caption-title'>The aggregation of SCO4226 triggered by nickel.</div>
Lu M, Jiang YL, Wang S, Jin H, Zhang RG, Virolle MJ, Chen Y, Zhou CZ
OpenAccess logo PLoS One 9 e109660 (2014)
Cited: 6 times
EuropePMC logo PMID: 25285530

Abstract

The open reading frame SCO4226 of Streptomyces coelicolor A3(2) encodes an 82-residue hypothetical protein. Biochemical assays revealed that each SCO4226 dimer binds four nickel ions. To decipher the molecular function, we solved the crystal structures of SCO4226 in both apo- and nickel-bound (Ni-SCO4226) forms at 1.30 and 2.04 Å resolution, respectively. Each subunit of SCO4226 dimer adopts a canonical ferredoxin-like fold with five β-strands flanked by two α-helices. In the structure of Ni-SCO4226, four nickel ions are coordinated at the surface of the dimer. Further biochemical assays suggested that the binding of Ni2+ triggers the self-aggregation of SCO4226 in vitro. In addition, RT-qPCR assays demonstrated that the expression of SCO4226 gene in S. coelicolor is specifically up-regulated by the addition of Ni2+, but not other divalent ions such as Cu2+, Mn2+ or Co2+. All these results suggested that SCO4226 acts as a nickel binding protein, probably required for nickel sequestration and/or detoxification.

Articles - 4oi3 mentioned but not cited (1)

  1. Streptomyces coelicolor SCO4226 is a nickel binding protein. Lu M, Jiang YL, Wang S, Jin H, Zhang RG, Virolle MJ, Chen Y, Zhou CZ. PLoS ONE 9 e109660 (2014)


Reviews citing this publication (3)

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  2. Heavy Metal Removal by Bioaccumulation Using Genetically Engineered Microorganisms. Diep P, Mahadevan R, Yakunin AF. Front Bioeng Biotechnol 6 157 (2018)
  3. Potential Application of Living Microorganisms in the Detoxification of Heavy Metals. Chen R, Tu H, Chen T. Foods 11 1905 (2022)

Articles citing this publication (2)

  1. Regulation of a nickel-cobalt efflux system and nickel homeostasis in a soil actinobacterium Streptomyces coelicolor. Kim HM, Ahn BE, Lee JH, Roe JH. Metallomics 7 702-709 (2015)
  2. Deciphering the Transcriptional Response Mediated by the Redox-Sensing System HbpS-SenS-SenR from Streptomycetes. Busche T, Winkler A, Wedderhoff I, Rückert C, Kalinowski J, Ortiz de Orué Lucana D. PLoS ONE 11 e0159873 (2016)


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