› 4gxt

X-ray diffraction
1.82Å resolution

The crystal structure of a conserved functionally unknown protein from Anaerococcus prevotii DSM 20548

Released:
DOI: 10.2210/pdb4gxt/pdb
PDB entry 4gxt coloured by chain, front view.
PDB entry 4gxt coloured by chain, side view.
PDB entry 4gxt coloured by chain, top view.
Source organism: Anaerococcus prevotii DSM 20548
Entry authors: Tan K, Li H, Bearden J, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
O-phospho-L(or D)-serine + H(2)O = L(or D)-serine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111758 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
phosphoserine phosphatase Chain: A
Molecule details ›
Chain: A
Length: 385 amino acids
Theoretical weight: 45.71 KDa
Source organism: Anaerococcus prevotii DSM 20548
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C7RF86 (Residues: 1-382; Coverage: 100%)
Gene name: Apre_0094
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 11 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 49.13Å b: 53.226Å c: 75.849Å
α: 90° β: 95.03° γ: 90°
R-values:
R R work R free
0.176 0.174 0.223
Expression system: Escherichia coli BL21(DE3)

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