4ewl

X-ray diffraction
1.85Å resolution

Crystal Structure of MshB with glycerol and Acetate bound in the active site

Released:

Function and Biology Details

Reaction catalysed:
1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + H(2)O = 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + acetate
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1D-myo-inositol 2-acetamido-2-deoxy-alpha-D-glucopyranoside deacetylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 299 amino acids
Theoretical weight: 31.46 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WJN3 (Residues: 1-299; Coverage: 99%)
Gene names: Rv1170, mshB
Sequence domains: GlcNAc-PI de-N-acetylase
Structure domains: LmbE-like

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: C2
Unit cell:
a: 147.379Å b: 71.903Å c: 97.681Å
α: 90° β: 128.29° γ: 90°
R-values:
R R work R free
0.191 0.189 0.224
Expression system: Escherichia coli BL21(DE3)