4cei

X-ray diffraction
2.8Å resolution

Crystal structure of ADPNP-bound AddAB with a forked DNA substrate

Released:
Entry authors: Krajewski WW, Wilkinson M, Fu X, Cronin NB, Wigley D

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (3 distinct):
ATP-dependent helicase/nuclease subunit A Chain: A
ATP-dependent helicase/deoxyribonuclease subunit B Chain: B
Molecule details ›
Chain: B
Length: 1166 amino acids
Theoretical weight: 134.77 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli B
UniProt:
  • Canonical: P23477 (Residues: 1-1166; Coverage: 100%)
Gene names: BSU10620, addB
Sequence domains:
Structure domains:
DNA Chain: X
Molecule details ›
Chain: X
Length: 65 nucleotides
Theoretical weight: 19.96 KDa

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 75.944Å b: 151.335Å c: 124.56Å
α: 90° β: 95.95° γ: 90°
R-values:
R R work R free
0.198 0.195 0.24
Expression system: Escherichia coli B