X-ray diffraction
2.6Å resolution

Crystal structure of unphosphorylated Maternal Embryonic Leucine zipper Kinase (MELK) in complex with AMP-PNP

Source organism: Homo sapiens
Entry authors: Canevari G, Re-Depaolini S, Cucchi U, Forte B, Carpinelli P, Bertrand JA

Function and Biology Details

Reactions catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-172082 (preferred)
Entry contents:
1 distinct polypeptide molecule
Maternal embryonic leucine zipper kinase Chain: A
Molecule details ›
Chain: A
Length: 347 amino acids
Theoretical weight: 40.22 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
  • Canonical: Q14680 (Residues: 2-340; Coverage: 52%)
Gene names: KIAA0175, MELK
Sequence domains:
Structure domains: Transferase(Phosphotransferase) domain 1

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 60.02Å b: 63.81Å c: 92.05Å
α: 90° β: 90° γ: 90°
R R work R free
0.184 0.18 0.26
Expression system: Spodoptera frugiperda