› 4gkw

X-ray diffraction
3.3Å resolution

Crystal Structure of the Coiled-coil Domain of C. elegans SAS-6

Released:
DOI: 10.2210/pdb4gkw/pdb
PDB entry 4gkw coloured by chain, front view.
PDB entry 4gkw coloured by chain, side view.
PDB entry 4gkw coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
SAS-6 coiled-coil structure and interaction with SAS-5 suggest a regulatory mechanism in C. elegans centriole assembly.
Qiao R, Cabral G, Lettman MM, Dammermann A, Dong G
EMBO J 31 4334-47 (2012)
PMID: 23064147

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130256 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spindle assembly abnormal protein 6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 167 amino acids
Theoretical weight: 19.36 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Best match: O62479 (Residues: 248-410)
Gene names: Y45F10D.9, sas-6

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P61
Unit cell:
a: 140.29Å b: 140.29Å c: 74.67Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.27 0.258 0.299
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Coiled-coils: The long and short of it.
Truebestein et al. (2016)
8 more

7 mentions without citation

SAS-6 coiled-coil structure and interaction with SAS-5 suggest a regulatory mechanism in C. elegans centriole assembly.
Qiao et al. (2012)
6 more