3u8p

X-ray diffraction
2.75Å resolution

Cytochrome b562 integral fusion with EGFP

Released:
Entry authors: Arpino J, Czapinska H, Piasecka A, Edwards WR, Barker P, Gajda M, Bochtler M, Jones DD

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein; Soluble cytochrome b562 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 347 amino acids
Theoretical weight: 38.98 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
  • Canonical: P42212 (Residues: 2-38, 40-238; Coverage: 99%)
Gene names: GFP, cybC
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 64.752Å b: 125.2Å c: 89.255Å
α: 90° β: 90.37° γ: 90°
R-values:
R R work R free
0.203 0.201 0.242
Expression system: Escherichia coli BL21(DE3)