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X-ray diffraction
2.1Å resolution

Crystal structure of bifunctional folylpolyglutamate synthase/dihydrofolate synthase complexed with AMPPNP and Mn ion from Yersinia pestis c092

Released:
DOI: 10.2210/pdb3pyz/pdb
PDB entry 3pyz coloured by chain, front view.
PDB entry 3pyz coloured by chain, side view.
PDB entry 3pyz coloured by chain, top view.
Source organism: Yersinia pestis CO92
Entry authors: Nocek B, Makowska-Grzyska M, Maltseva N, Anderson W, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reactions catalysed: 
ATP + 7,8-dihydropteroate + L-glutamate = ADP + phosphate + 7,8-dihydropteroylglutamate
ATP + tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + tetrahydropteroyl-(gamma-Glu)(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184034 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate synthase/folylpolyglutamate synthase Chain: A
Molecule details ›
Chain: A
Length: 437 amino acids
Theoretical weight: 47.55 KDa
Source organism: Yersinia pestis CO92
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8D0U0 (Residues: 27-460; Coverage: 94%)
Gene names: YP_2395, folC, y1602
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ANP 1 x ANP
Ligand MN 1 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 60.627Å b: 77.713Å c: 122.292Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.176 0.228
Expression system: Escherichia coli BL21

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