› 3ot4

X-ray diffraction
2.4Å resolution

Structure and Catalytic Mechanism of Bordetella bronchiseptica nicF

Released:
DOI: 10.2210/pdb3ot4/pdb
PDB entry 3ot4 coloured by chain, front view.
PDB entry 3ot4 coloured by chain, side view.
PDB entry 3ot4 coloured by chain, top view.
Source organism: Bordetella bronchiseptica
Entry authors: Rowlett RS, Snider MJ

Function and Biology Details

Reaction catalysed: 
Maleamate + H(2)O = maleate + NH(3)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102082 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isochorismatase-like domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 236 amino acids
Theoretical weight: 25.27 KDa
Source organism: Bordetella bronchiseptica
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H3LKK8 (Residues: 1-205; Coverage: 100%)
Gene names: BB0275, BB1782
Sequence domains: Isochorismatase family
Structure domains: Isochorismatase-like

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CSO 8 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: OXFORD DIFFRACTION ENHANCE ULTRA
Spacegroup: R3
Unit cell:
a: 157.56Å b: 157.56Å c: 198.48Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.242 0.241 0.311
Expression system: Escherichia coli

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