3net

X-ray diffraction
2.7Å resolution

Crystal structure of histidyl-tRNA synthetase from Nostoc sp. PCC 7120

Released:
Source organism: Nostoc sp. PCC 7120
Entry authors: Chang C, Bigelow L, Carroll J, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed:
ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histidine--tRNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 465 amino acids
Theoretical weight: 52.13 KDa
Source organism: Nostoc sp. PCC 7120
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8YMC2 (Residues: 1-462; Coverage: 100%)
Gene names: all5012, hisS
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P4322
Unit cell:
a: 73.656Å b: 73.656Å c: 521.912Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.217 0.265
Expression system: Escherichia coli