› 3lun

X-ray diffraction
1.8Å resolution

Structure of ulilysin mutant M290C

Released:
DOI: 10.2210/pdb3lun/pdb
PDB entry 3lun coloured by chain, front view.
PDB entry 3lun coloured by chain, side view.
PDB entry 3lun coloured by chain, top view.
Source organism: Methanosarcina acetivorans
Primary publication:
On the relevance of the Met-turn methionine in metzincins.
Tallant C, García-Castellanos R, Baumann U, Gomis-Rüth FX
J Biol Chem 285 13951-7 (2010)
PMID: 20202937

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-186282 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ulilysin Chains: A, B
Molecule details ›
Chains: A, B
Length: 262 amino acids
Theoretical weight: 29.01 KDa
Source organism: Methanosarcina acetivorans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TL28 (Residues: 61-322; Coverage: 77%)
Gene name: MA_3214
Sequence domains: Pregnancy-associated plasma protein-A
Structure domains: Collagenase (Catalytic Domain)

Ligands and Environments

5 bound ligands:
Ligand ZN 2 x ZN
Ligand CA 6 x CA
Ligand GOL 4 x GOL
Ligand ARG 1 x ARG
Ligand VAL 1 x VAL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2221
Unit cell:
a: 58.51Å b: 116.29Å c: 164.76Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.161 0.208
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Architecture and function of metallopeptidase catalytic domains.
Cerdà-Costa et al. (2014)
3 more

1 mention without citation

On the relevance of the Met-turn methionine in metzincins.
Tallant et al. (2010)