› 3l4i

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF THE N-TERMINAL DOMAIN OF HSP70 (CGD2_20) FROM Cryptosporidium PARVUM IN COMPLEX WITH ADP and inorganic phosphate

Released:
DOI: 10.2210/pdb3l4i/pdb
PDB entry 3l4i coloured by chain, front view.
PDB entry 3l4i coloured by chain, side view.
PDB entry 3l4i coloured by chain, top view.
Source organism: Cryptosporidium parvum Iowa II
Entry authors: Pizarro JC, Wernimont AK, Hutchinson A, Sullivan H, Lew J, Bochkarev A, Arrowsmith CH, Bountra C, Weigelt J, Edwards AM, Hui R, Hills T, Structural Genomics Consortium (SGC)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176912 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heat shock 70 kDa protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 400 amino acids
Theoretical weight: 44.23 KDa
Source organism: Cryptosporidium parvum Iowa II
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5CPP8 (Residues: 15-396; Coverage: 56%)
Gene name: cgd2_20
Sequence domains: Hsp70 protein
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ADP 2 x ADP
Ligand PO4 2 x PO4
Ligand EDO 2 x EDO
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: C2221
Unit cell:
a: 85.977Å b: 115.324Å c: 182.314Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.252
Expression system: Escherichia coli BL21(DE3)

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