› 3kuu

X-ray diffraction
1.41Å resolution

Structure of the PurE Phosphoribosylaminoimidazole Carboxylase Catalytic Subunit from Yersinia pestis

Released:
DOI: 10.2210/pdb3kuu/pdb
PDB entry 3kuu coloured by chain, front view.
PDB entry 3kuu coloured by chain, side view.
PDB entry 3kuu coloured by chain, top view.
Source organism: Yersinia pestis
Entry authors: Anderson SM, Wawrzak Z, Brunzelle JS, Onopriyenko O, Kwon K, Edwards A, Savchenko A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-104502 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N5-carboxyaminoimidazole ribonucleotide mutase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 174 amino acids
Theoretical weight: 18.24 KDa
Source organism: Yersinia pestis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A2S9PDM1 (Residues: 1-174; Coverage: 100%)
Gene names: YPO3076, purE
Sequence domains: AIR carboxylase
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand SO4 14 x SO4
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: I422
Unit cell:
a: 114.04Å b: 114.04Å c: 231.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.151 0.15 0.167
Expression system: Escherichia coli BL21(DE3)

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