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Structural models for interactions between the 20S proteasome and its PAN/19S activators.

Stadtmueller BM, Ferrell K, Whitby FG, Heroux A, Robinson H, Myszka DG, Hill CP
J Biol Chem 285 13-7 (2010)
Related entries: 3jrm, 3jse

Cited: 47 times
EuropePMC logo PMID: 19889631

Abstract

Proteasome activity is regulated by sequestration of its proteolytic centers in a barrel-shaped structure that limits substrate access. Substrates enter the proteasome by means of activator complexes that bind to the end rings of proteasome alpha subunits and induce opening of an axial entrance/exit pore. The PA26 activator binds in a pocket on the proteasome surface using main chain contacts of its C-terminal residues and uses an internal activation loop to trigger gate opening by repositioning the proteasome Pro-17 reverse turn. Subunits of the unrelated PAN/19S activators bind with their C termini in the same pockets but can induce proteasome gate opening entirely from interactions of their C-terminal peptides, which are reported to cause gate opening by inducing a rocking motion of proteasome alpha subunits rather than by directly contacting the Pro-17 turn. Here we report crystal structures and binding studies of proteasome complexes with PA26 constructs that display modified C-terminal residues, including those corresponding to PAN. These findings suggest that PA26 and PAN/19S C-terminal residues bind superimposably and that both classes of activator induce gate opening by using direct contacts to residues of the proteasome Pro-17 reverse turn. In the case of the PAN and 19S activators, a penultimate tyrosine/phenylalanine residue contacts the proteasome Gly-19 carbonyl oxygen to stabilize the open conformation.

Articles - 3jtl mentioned but not cited (2)

  1. Structural models for interactions between the 20S proteasome and its PAN/19S activators. Stadtmueller BM, Ferrell K, Whitby FG, Heroux A, Robinson H, Myszka DG, Hill CP. J Biol Chem 285 13-17 (2010)
  2. Crystal structure of a low molecular weight activator Blm-pep with yeast 20S proteasome - insights into the enzyme activation mechanism. Witkowska J, Giżyńska M, Grudnik P, Golik P, Karpowicz P, Giełdoń A, Dubin G, Jankowska E. Sci Rep 7 6177 (2017)


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  1. Molecular architecture and assembly of the eukaryotic proteasome. Tomko RJ, Hochstrasser M. Annu Rev Biochem 82 415-445 (2013)
  2. Proteasome activators. Stadtmueller BM, Hill CP. Mol Cell 41 8-19 (2011)
  3. Structural biology of the proteasome. Kish-Trier E, Hill CP. Annu Rev Biophys 42 29-49 (2013)
  4. The 26S proteasome: assembly and function of a destructive machine. Gallastegui N, Groll M. Trends Biochem Sci 35 634-642 (2010)
  5. Systemic and renal lipids in kidney disease development and progression. Wahl P, Ducasa GM, Fornoni A, Fornoni A. Am J Physiol Renal Physiol 310 F433-45 (2016)
  6. Structure characterization of the 26S proteasome. Kim HM, Yu Y, Cheng Y. Biochim Biophys Acta 1809 67-79 (2011)
  7. Regulation of lamin properties and functions: does phosphorylation do it all? Machowska M, Piekarowicz K, Rzepecki R. Open Biol 5 150094 (2015)
  8. Structure, Dynamics and Function of the 26S Proteasome. Mao Y. Subcell Biochem 96 1-151 (2021)
  9. Assembly manual for the proteasome regulatory particle: the first draft. Park S, Tian G, Roelofs J, Finley D. Biochem Soc Trans 38 6-13 (2010)
  10. Allosteric effects in the regulation of 26S proteasome activities. Sledź P, Förster F, Baumeister W. J Mol Biol 425 1415-1423 (2013)
  11. Emerging mechanistic insights into AAA complexes regulating proteasomal degradation. Förster F, Schuller JM, Unverdorben P, Aufderheide A. Biomolecules 4 774-794 (2014)
  12. Small-Molecule Inhibitors of the Proteasome's Regulatory Particle. Muli CS, Tian W, Trader DJ. Chembiochem 20 1739-1753 (2019)

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  1. Dynamic regulation of archaeal proteasome gate opening as studied by TROSY NMR. Religa TL, Sprangers R, Kay LE. Science 328 98-102 (2010)
  2. Structure of a Blm10 complex reveals common mechanisms for proteasome binding and gate opening. Sadre-Bazzaz K, Whitby FG, Robinson H, Formosa T, Hill CP. Mol Cell 37 728-735 (2010)
  3. Structural basis for dynamic regulation of the human 26S proteasome. Chen S, Wu J, Lu Y, Ma YB, Lee BH, Yu Z, Ouyang Q, Finley DJ, Kirschner MW, Mao Y. Proc Natl Acad Sci U S A 113 12991-12996 (2016)
  4. Identification of the Cdc48•20S proteasome as an ancient AAA+ proteolytic machine. Barthelme D, Sauer RT. Science 337 843-846 (2012)
  5. Rpn1 and Rpn2 coordinate ubiquitin processing factors at proteasome. Rosenzweig R, Bronner V, Zhang D, Fushman D, Glickman MH. J Biol Chem 287 14659-14671 (2012)
  6. Proteasome allostery as a population shift between interchanging conformers. Ruschak AM, Kay LE. Proc Natl Acad Sci U S A 109 E3454-62 (2012)
  7. An asymmetric interface between the regulatory and core particles of the proteasome. Tian G, Park S, Lee MJ, Huck B, McAllister F, Hill CP, Gygi SP, Finley D. Nat Struct Mol Biol 18 1259-1267 (2011)
  8. Loss of Rpt5 protein interactions with the core particle and Nas2 protein causes the formation of faulty proteasomes that are inhibited by Ecm29 protein. Lee SY, De la Mota-Peynado A, Roelofs J. J Biol Chem 286 36641-36651 (2011)
  9. Structural mechanism for nucleotide-driven remodeling of the AAA-ATPase unfoldase in the activated human 26S proteasome. Zhu Y, Wang WL, Yu D, Ouyang Q, Lu Y, Mao Y. Nat Commun 9 1360 (2018)
  10. Architecture and assembly of the archaeal Cdc48*20S proteasome. Barthelme D, Chen JZ, Grabenstatter J, Baker TA, Sauer RT. Proc Natl Acad Sci U S A 111 E1687-94 (2014)
  11. Structure of a proteasome Pba1-Pba2 complex: implications for proteasome assembly, activation, and biological function. Stadtmueller BM, Kish-Trier E, Ferrell K, Petersen CN, Robinson H, Myszka DG, Eckert DM, Formosa T, Hill CP. J Biol Chem 287 37371-37382 (2012)
  12. Cryo-EM structures of the archaeal PAN-proteasome reveal an around-the-ring ATPase cycle. Majumder P, Rudack T, Beck F, Danev R, Pfeifer G, Nagy I, Baumeister W. Proc Natl Acad Sci U S A 116 534-539 (2019)
  13. Molecular and cellular roles of PI31 (PSMF1) protein in regulation of proteasome function. Li X, Thompson D, Kumar B, DeMartino GN. J Biol Chem 289 17392-17405 (2014)
  14. The proteasome-associated protein Ecm29 inhibits proteasomal ATPase activity and in vivo protein degradation by the proteasome. De La Mota-Peynado A, Lee SY, Pierce BM, Wani P, Singh CR, Roelofs J. J Biol Chem 288 29467-29481 (2013)
  15. Proteasomal degradation of Sfp1 contributes to the repression of ribosome biogenesis during starvation and is mediated by the proteasome activator Blm10. Lopez AD, Tar K, Krügel U, Dange T, Ros IG, Schmidt M. Mol Biol Cell 22 528-540 (2011)
  16. The archaeal proteasome is regulated by a network of AAA ATPases. Forouzan D, Ammelburg M, Hobel CF, Ströh LJ, Sessler N, Martin J, Lupas AN. J Biol Chem 287 39254-39262 (2012)
  17. ATP binding by proteasomal ATPases regulates cellular assembly and substrate-induced functions of the 26 S proteasome. Kim YC, Li X, Thompson D, DeMartino GN. J Biol Chem 288 3334-3345 (2013)
  18. C termini of proteasomal ATPases play nonequivalent roles in cellular assembly of mammalian 26 S proteasome. Kim YC, DeMartino GN. J Biol Chem 286 26652-26666 (2011)
  19. Small Molecule Modulation of Proteasome Assembly. Njomen E, Osmulski PA, Jones CL, Gaczynska M, Tepe JJ. Biochemistry 57 4214-4224 (2018)
  20. The C terminus of Rpt3, an ATPase subunit of PA700 (19 S) regulatory complex, is essential for 26 S proteasome assembly but not for activation. Kumar B, Kim YC, DeMartino GN. J Biol Chem 285 39523-39535 (2010)
  21. Immune responses induced by oligogalacturonides are differentially affected by AvrPto and loss of BAK1/BKK1 and PEPR1/PEPR2. Gravino M, Locci F, Tundo S, Cervone F, Savatin DV, De Lorenzo G. Mol Plant Pathol 18 582-595 (2017)
  22. Proline- and Arginine-Rich Peptides as Flexible Allosteric Modulators of Human Proteasome Activity. Giżyńska M, Witkowska J, Karpowicz P, Rostankowski R, Chocron ES, Pickering AM, Osmulski P, Gaczynska M, Jankowska E. J Med Chem 62 359-370 (2019)
  23. Stable incorporation of ATPase subunits into 19 S regulatory particle of human proteasome requires nucleotide binding and C-terminal tails. Lee SH, Moon JH, Yoon SK, Yoon JB. J Biol Chem 287 9269-9279 (2012)
  24. Cryo-EM structures of the human PA200 and PA200-20S complex reveal regulation of proteasome gate opening and two PA200 apertures. Guan H, Wang Y, Yu T, Huang Y, Li M, Saeed AFUH, Perčulija V, Li D, Xiao J, Wang D, Zhu P, Ouyang S. PLoS Biol 18 e3000654 (2020)
  25. Novel Rosaceae plant elicitor peptides as sustainable tools to control Xanthomonas arboricola pv. pruni in Prunus spp. Ruiz C, Nadal A, Montesinos E, Pla M. Mol Plant Pathol 19 418-431 (2018)
  26. N-terminal α7 deletion of the proteasome 20S core particle substitutes for yeast PI31 function. Yashiroda H, Toda Y, Otsu S, Takagi K, Mizushima T, Murata S. Mol Cell Biol 35 141-152 (2015)
  27. The YΦ motif defines the structure-activity relationships of human 20S proteasome activators. Opoku-Nsiah KA, de la Pena AH, Williams SK, Chopra N, Sali A, Lander GC, Gestwicki JE. Nat Commun 13 1226 (2022)
  28. [Proteasome activity in tumors of female reproductive system]. Kondakova IV, Spirina LV, Shashova EE, Koval' VD, Kolomiets LA, Chernysheva AL, Slonimskaia EM. Bioorg Khim 38 106-110 (2012)
  29. Tagging the proteasome active site β5 causes tag specific phenotypes in yeast. Waite KA, Burris A, Roelofs J. Sci Rep 10 18133 (2020)
  30. Minimal mechanistic component of HbYX-dependent proteasome activation that reverses impairment by neurodegenerative-associated oligomers. Chuah JJY, Thibaudeau TA, Smith DM. Commun Biol 6 725 (2023)
  31. High resolution structures define divergent and convergent mechanisms of archaeal proteasome activation. Chuah JJY, Rexroad MS, Smith DM. Commun Biol 6 733 (2023)
  32. Mechanism of proteasome gate modulation by assembly chaperones Pba1 and Pba2. Schnell HM, Ang J, Rawson S, Walsh RM, Micoogullari Y, Hanna J. J Biol Chem 298 101906 (2022)
  33. Orthogonal approaches required to measure proteasome composition and activity in mammalian brain tissue. Türker F, Bharadwaj RA, Kleinman JE, Weinberger DR, Hyde TM, White CJ, Williams DW, Margolis SS. J Biol Chem 299 104811 (2023)


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